Study type: Medical/biological study (experimental study)

Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG-63 and Saos-2) med./bio.

By: Santini MT, Rainaldi G, Ferrante A, Indovina PL, Vecchia P, Donelli G
Published in: Bioelectromagnetics 2003; 24 (5): 327-338
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Aim of study (acc. to author)

To study the possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules in two human osteosarcoma cell lines.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 7 days
Exposure 2: 50 Hz
Exposure duration: continuous for 14 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 7 days
Exposure setup
Exposure source
Chamber Incubator maintained at 37°C.
Setup 25 cm² tissue culture flask kept in the incubator. The coils were also located in the incubator.
Additional info Sham exposures were performed in the same incubator but inside a µ-metal cylinder(30 cm long x 23.5 cm diameter) with AC MF 0.84 µT and static component 0.22 µT. Control sample separate incubator with ambient AC MF 0.36 µT and static MF 40.2 µT.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT peak value measured - -
Additional parameter details

MF horizontal w.r.t gravity and cells. Static MF measured to be 43.8 µT.

Measurement and calculation details

The magnetic field was measured with a EFA-2 field analyzer and a 3 cm diameter probe. Static magnetic field was measured with a Hall effect meter and a transverse field probe.

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 14 days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT peak value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data demonstrated that no variations in cell surface morphology and cell death occurred between control and exposed cells in both cell lines, while significant changes were revealed in cell growth and fibronectin and CD44 expression in MG-63 cells.
The findings are discussed in view of the important role that cell adhesion molecules play in controlling various cancer cell functions, particularly proliferation and metastasis.

Study character:

Study funded by

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