Study type: Medical/biological study (experimental study)

Proflavin and microwave radiation: absence of a mutagenic interaction med./bio.

By: Meltz ML, Eagan P, Erwin DN
Published in: Bioelectromagnetics 1990; 11 (2): 149-157
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Aim of study (acc. to author)

To investigate whether simultaneous exposure of mouse leukemic cells to radiofrequency radiation at moderate power levels can affect the extent of mutagenesis caused by proflavine (a drug known to intercalate with DNA).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: pulsed
Exposure duration: continuous for 4 h

General information

Six exposure conditions: 37°C control, radio frequency exposure, temperature control exposure, proflavin treatment at 37°C, proflavin treatment with simultaneous radio frequency exposure and proflavin treatment with simultaneous temperature control.

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Waveform
Exposure duration continuous for 4 h
Modulation
Modulation type pulsed
Pulse width 10 µs
Duty cycle 0.25 %
Packets per second 25,000
Repetition frequency 1 Hz
Exposure setup
Exposure source
Distance between exposed object and exposure source 1.6 m
Chamber anechoic chamber / 12 m x 6 m x 3 m
Setup One bath was placed directly beneath the horn
Additional info T-25 flasks were placed on the underside of the circular Styrofoam wheel, which was placed into a water bath. Two other water baths (at 37°C and for TC) were located at the far end of the anechoic chamber.
Parameters
Measurand Value Type Method Mass Remarks
power density 870 W/m² unspecified measured - in Experiment 1 and 2.
power density 650 W/m² unspecified measured - in Experiment 3.
SAR 40 mW/g mean calculated unspecified in Experiment 3.
SAR 40.8 mW/g mean calculated unspecified in experiment 1 and 2, standard deviation of +/- 13.4 W/kg.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In no case any indication of a statistically significant increase in the induced mutant frequency due to simultaneous exposure to radiofrequency radiation (RFR) and proflavine was found, as compared with the proflavine exposures alone. There was also no indication of any change in the colony-size distribution of the resulting mutant colonies, neigther. And there was no evidence in these experiments of any mutagenic action by the RFR alone.

Study character:

Study funded by

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