The effects of exposure to a 50 Hz or 120 Hz magnetic field on steroidogenic activity of myometrium collected from pigs should be investigated.
Myometrial samples were collected from female pigs after 15-16 of pregnancy and divided into the following groups: 1) exposure to the 50 Hz magnetic field for 2 hours, 2) exposure to the 120 Hz magnetic field for 2 hours, 3) control group for 2 hour-exposure, 4) exposure to the 50 Hz magnetic field for 4 hours, 5) exposure to the 120 Hz magnetic field for 4 hours, 6) control group for 4 hour-exposure. To determine whether progesterone functions as a protectant from magnetic field exposure, some samples were treated with progesterone.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
2 or 4 hours
|
|
|
Exposure 2:
120 Hz
Exposure duration:
2 or 4 hours
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 2 or 4 hours |
| Exposure source |
|
|---|---|
| Distance between exposed object and exposure source | 5 cm |
| Chamber | 24-well plates |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 8 mT | - | - | - | - |
| Frequency | 120 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 2 or 4 hours |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 8 mT | - | - | - | - |
Without progesterone treatment, exposure to the 50 Hz magnetic field (group 1) and 120 Hz magnetic field for 2 hours (group 2) significantly decreased androstenedione release compared to the control group. With progesterone treatment, testosterone release was signifantly decreased compared to the control group. Exposure to the 50 Hz magnetic field significantly increased the gene expression of HSD3B1 and level of cytochrome P450c17 after 4 hours of exposure (group 4) without progesterone treatment compared to the control group. Exposure to the 120 Hz magnetic field for 4 hours (group 5) significantly decreased androstenedione release compared to the control group without progesterone treatment. With progesterone treatment, CYP17A1 gene expression was significantly decreased and the level of 3βHSD and androstenedione release were significantly increased compared to the control group.
The authors conclude that exposure to a 50 Hz or 120 Hz magnetic field might alter steroidogenic activity of myometrium collected from pigs without an influence of progesterone.
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