Study type: Medical/biological study (experimental study)

Exposure to 915 MHz radiation induces micronuclei in Vicia faba root tips med./bio.

Published in: Mutagenesis 2016; 31 (2): 187-192

Aim of study (acc. to author)

The genotoxic effects of exposure of broad bean seedlings (Vicia faba) to a 915 MHz electromagnetic field should be investigated.

Background/further details

Seedlings were divided into the following groups: exposure to a 1) 1.5 W, 2) 2.3 W and 3) 3 W electromagnetic field and 4) sham exposure. A positive control was conducted.
Three independent experiments were performed and in each experiment 15 root tips and 5000 cells/tip were investigated in each group by different operators.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 915 MHz
Modulation type: CW
Exposure duration: continuous for 72 hours
Exposure 2: 915 MHz
Modulation type: CW
Exposure duration: continuous for 72 hours
Exposure 3: 915 MHz
Modulation type: CW
Exposure duration: continuous for 72 hours

Exposure 1

Main characteristics
Frequency 915 MHz
Type
Exposure duration continuous for 72 hours
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber plastic containers (101 Œ 74 mm)
Setup TEM was made of copper clad FR-4 plates; the sides of the cell were left open to simplify access; tapered walls were used to join the central region of the cell, 450 mm long, to the input and output coaxial connectors; whole system was kept inside a thermostatic cabinet with a constant temperature of 20°C in the dark
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power 1.5 W mean measured - ± 0.1 W
power density 23 W/m² mean calculated - ± 1.5 W
SAR 0.4 W/kg minimum estimated - -

Exposure 2

Main characteristics
Frequency 915 MHz
Type
Exposure duration continuous for 72 hours
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power 2.3 W mean measured - ± 0.1 W
power density 35.2 W/m² mean calculated - ± 1.5 W

Exposure 3

Main characteristics
Frequency 915 MHz
Type
Exposure duration continuous for 72 hours
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power 3 W mean measured - ± 0.2 W
power density 46 W/m² mean calculated - ± 3.1W
SAR 1 W/kg minimum estimated - -
SAR 1.6 W/kg maximum estimated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The micronucleus frequency was significantly increased in all exposure groups (group 1-3) compared to the sham exposure group (group 4). The highest value was measured in group 3, showing a 7-fold higher value than the sham exposure group.
The authors conclude that exposure of broad bean seedlings (Vicia faba) to a 915 MHz electromagnetic field could have genotoxic effects.

Study character:

Study funded by

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