To explore biological and technical variables which could influence the effects of electromagnetic fields on c-myc gene expression in two HL60 cell populations.
The authors tried to find an explanation for the inability of two groups of investigators (see publication 919, publication 2068) to replicate their results (see publication 895, publication 920). The following criteria were further investigated: different HL60 cell populations (specific growth characteristics, response to the positive control TPA treatment, c-myc transcript level), mRNA extraction procedure (SDS/phenol versus guanidine/phenol), the stability/variability of internal standards, sham exposure set-up (double-wound coils versus single-wound coils), shielding effectiveness.
Exposure | Parameters |
---|---|
Exposure 1:
60 Hz
Exposure duration:
20 min
field of one coil
|
|
Exposure 2:
60 Hz
Exposure duration:
20 min
field of two coils
|
|
Frequency | 60 Hz |
---|---|
Type | |
Waveform | |
Exposure duration | 20 min |
Additional info | field of one coil |
Exposure source | |
---|---|
Chamber | shielding of the coils by cylindrical mu-metal box (30 cm high x 15 cm in radius, 0.040" thick) with a shielding factor of 39 dB |
Setup | a single pair of Helmholtz coils ( 13 cm x 14 cm ) with 164 turns of copper wire, separated by 8 cm, T-25 flasks including the cells kept on a Plexiglass platform inside the coils |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
magnetic flux density | 8 µT | peak value | measured | - | - |
Frequency | 60 Hz |
---|---|
Type | |
Waveform | |
Exposure duration | 20 min |
Additional info | field of two coils |
Exposure source |
|
---|---|
Setup | double wound Helmholtz coils ( 13 cm x 14 cm ) with 164 turns of copper wire, separated by 8 cm |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
magnetic flux density | 8 µT | peak value | measured | - | - |
Different c-myc level and cell growth after exposure between the two investigated cell populations were observed.
The response to the positive control TPA differed as well.
The two compared RNA extraction procedures showed different c-myc RNA levels.
There were no differences in the stability of both internal standards and the examined sham exposure set-ups.
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