Medical/biological study (experimental study)

Detection of oxidative stress induced by mobile phone radiation in tissues of mice using 8-oxo-7, 8-dihydro-20-deoxyguanosine as a biomarker med./bio.

By: Khalil AM, Alshamali AM, Gagaa MH

Published in: World Acad Sci Eng Technol 2011; 5 (4): 657-622

Aim of study (acc. to author)

To study the extent of radiofrequency induced oxidative DNA damage by measuring the level of 8-hydroxy-2-deoxyguanosine in mice tissues after 900 MHz mobile phone exposure.

Background/further details

36 mice were divided into an exposure group and a sham exposure group (each group n=18; with three subgroups of six mice each). Tissue samples were taken 24 h after exposure.

Endpoint

genotoxicity/mutation: DNA damage/oxidative stress (levels of 8-hydroxy-2-deoxyguanosine in serum, brain and spleen)

Exposure

- 900 MHz
- RF
- mobile communications
- digital mobile phone
- GSM

Exposure	Parameters
Exposure 1: 900 MHz Exposure duration: 30 min/day for 30 days	SAR: 1 W/kg average over mass (whole body) (equivalent to 25 V/m)

Exposure 1

Main characteristics

Frequency	900 MHz
Type	electromagnetic field
Exposure duration	30 min/day for 30 days

Exposure setup

Exposure source	GSM signal generator
Chamber	mice placed randomly in groups of six animals in plastic cages exposed to the mobile phone fields
Setup	the antenna was located about 5 cm next to the cage
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
SAR	1 W/kg	average over mass	measured and calculated	whole body	equivalent to 25 V/m

Additional parameter details

radiofrequency field adjusted to 25 V/m, simulating the actual exposure levels from current GSM mobile phones to the direction of human tissues

Measurement and calculation details

High Frequency Structure Simulator (HFSS) software used to simulate the exposure environment

Exposed system:

animal
mouse/BALB/c
whole body

Methods Endpoint/measurement parameters/methodology

genotoxicity/mutation: DNA damage/oxidative stress (levels of 8-hydroxy-2-deoxyguanosine in serum, brain and spleen; ELISA)
body weight, mortality, morbidity

Investigated system:

DNA/RNA
tissue homogenates
investigation on living organism

Investigated organ system:

Time of investigation:

before exposure
after exposure

Main outcome of study (acc. to author)

All animals survived the whole experimental period and the body weight of the animals did not change significantly at the end of the experiment. Additionally, no statistically significant differences were found in the parameters of oxidative stress.
In conclusion, the data indicate that a 900 MHz radiofrequency exposure did not induce a significant oxidative DNA damage under these experimental conditions.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Yarmouk University, Irbid, Jordan

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