To study the effects of an electric field, low temperature, and their combinations on tissue vitality and some physiological variables regarding antioxidant responses of cold-sensitive bean and cold-tolerant kale leaves.
15 day old seedlings were exposed to an electric field prior to cold treatment (5/2°C day/night). Plants were harvested on 3rd and 6th day after electric field application.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
10 min. or 40 min.
|
|
Leaves were treated in six groups: i) control ii) 10 min. exposure iii) 40 min. exposure iv) cold treatment v) 10 min. exposure + cold treatment vi) 40 min. exposure + cold treatment
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 10 min. or 40 min. |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| electric field strength | 100 kV/m | - | calculated | - | - |
In both plants, cold application alone caused statistically significant increments in total protein levels and the antioxidant enzyme activities. However, tissue vitality and hydrogen peroxide levels did not change in the cold-tolerant kale, while tissue vitality decreased and hydrogen peroxide levels increased in cold-sensitive bean.
Electric field application alone did not cause any significant changes in the both plant species. On the other hand, 40 min electric field exposure increased the deteriorative effect of cold treatment in both plant species, while 10 min electric field augmented the chilling resistance by increasing the tissue vitality and antioxidant enzyme activities resulting in decreased hydrogen peroxide levels.
The authors conclude that electric field exposure (100 kV/m) for a very short time (10 min) before chilling treatment augments the chilling resistance by elevating the enzymatic antioxidant activation and tissue vitality, thus resulting in less hydrogen peroxide formation especially in cold-sensitive species.
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