To evaluate the effects of intermediate frequency magnetic fields generated by induction heating cooktops on cellular genotoxicity and stress responses.
Different cell lines were used for different endpoints. For positive controls, the cells were exposed to 2 Gy X rays (for cell growth), to bleomycin (for micronucleus formation), to ethylmethansulfonate (for HPRT assay), or to heat (for heat shock protein expression).
| Exposure | Parameters |
|---|---|
|
Exposure 1:
23 kHz
Exposure duration:
continuous for 2 hr
|
|
| Frequency | 23 kHz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 2 hr |
| Exposure source | |
|---|---|
| Setup | 35 cm high solenoid coil (outer diameter = 45 cm, inner diameter = 29 cm) with 96 turns of insulated copper wire; coil with cooling system placed inside an incubator; culture dishes stacked in four layers and positioned in the center of the coil in the 9.5 cm high exposure area of 10 cm diameter |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 6.05 mT | effective value | measured | - | - |
The data did not detect any effects of the intermediate frequency magnetic fields on cell growth, comet assay, micronucleus formation, HPRT gene mutation, expression of phosphorylated Hsp27, or nuclear translocation of Hsp27, Hsp70 or Hsp105. In conclusion, the data indicate that exposure to an intermediate frequency magnetic field at 6.05 mT(rms-value) for 2 h does not cause detectable cellular genotoxicity, and does not induce detectable cellular stress.
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