Study type: Medical/biological study (experimental study)

Intermediate frequency magnetic fields do not have mutagenic, co-mutagenic or gene conversion potentials in microbial genotoxicity tests med./bio.

By: Nakasono S, Ikehata M, Dateki M, Yoshie S, Shigemitsu T, Negishi T
Published in: Mutat Res Genet Toxicol Environ Mutagen 2008; 649 (1-2): 187-200
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Aim of study (acc. to author)

The purpose of this in vitro study was to evaluate the effects of intermediate frequency magnetic fields on mutagenicity and co-mutagenicity in bacteria and gene conversion in budding yeast cells.

Background/further details

For co-mutagenicity test bacteria were treated with five chemical mutagens: free radical precursor t-butyl hydroperoxide (BH), DNA reactive reagents 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF2) and N-ethyl-N-nitro-N-nitrosoguanidine (ENNG), benzpyrene and DNA reactive promutagen 2-aminoanthracene (2AA). As a negative control solvent was used in place of the mutagen solution. Positive controls were performed with known mutagens. Each experiment was repeated at least five times.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2 kHz
Exposure duration: continuous for 48 h
Exposure 2: 20 kHz
Exposure duration: continuous for 48 h
Exposure 3: 60 kHz
Exposure duration: continuous for 48 h

Exposure 1

Main characteristics
Frequency 2 kHz
Type
Waveform
Exposure duration continuous for 48 h
Exposure setup
Exposure source
Setup cells in plastic incubator between coils
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.91 mT effective value measured - -

Exposure 2

Main characteristics
Frequency 20 kHz
Type
Waveform
Exposure duration continuous for 48 h
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.1 mT effective value measured - -

Exposure 3

Main characteristics
Frequency 60 kHz
Type
Waveform
Exposure duration continuous for 48 h
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.11 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The exposure to intermediate frequency magnetic fields did not show statistically significant effects neihter in mutagenicity nor in co-mutagenicity bacterial test. In addition, no changes in gene conversion were found in yeast cells.

Study character:

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