Medical/biological study (experimental study)

Effects of extremely low-frequency magnetic fields on the oviposition of Drosophila melanogaster over three generations med./bio.

By: Gonet B, Kosik-Bogacka DI, Kuzna-Grygiel W

Published in: Bioelectromagnetics 2009; 30 (8): 687-689

Aim of study (acc. to author)

To study the effects of an extremely low frequency 50 Hz magnetic field on the oviposition and development of Drosophila melanogaster over three generations (parental generation, first filial generation (F1), second filial generation (F2), third filial generation (F3)).

Background/further details

For each generation, four control and four experimental colonies were established in parallel. Flies from experimental colonies of the parental generation and first filial generation (F1) were exposed to an extremely low frequency 50 Hz magnetic field (F2 and F3 were not exposed). The difference in temperature in the experimental and control groups did not exceed 1.5°C.

Endpoint

effects on embryo/fetus: development of Drosophila melanogaster
effects on the reproductive system: oviposition of Drosophila melanogaster

Exposure

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for two generations	magnetic flux density: 2 mT effective value (+/- 0.2 mT)

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for two generations

Exposure setup

Exposure source	Helmholtz coils
Setup	pair of Helmholtz coils with a diamter of 17 cm each, separated by 7 cm; flies in glass bottles or petri dishes placed in a cork-jacketed cylinder inside the coils

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	2 mT	effective value	measured	-	+/- 0.2 mT

Exposed system:

Methods Endpoint/measurement parameters/methodology

effects on embryo/fetus: development of Drosophila melanogaster (number of mature individuals)
effects on the reproductive system: oviposition of Drosophila melanogaster (number of eggs and number of eggs that did not develop; microscopy)

Investigated system:

eggs
investigation on living organism

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Exposed females of the parental generation laid 34% more eggs than the control females and the embryogenesis of these eggs was accelerated (first larvae of the F1 generation developed 24 h earlier than the control larvae). However, the data showed that exposure of Drosophila melanogaster females of parental generation and exposure of all the development stages of the first filial generation to an extremely low frequency magnetic field weakened the oviposition of these insects in their subsequent unexposed generations F2 and F3.

Study character:

medical/biological study
experimental study
full/main study
Brief Communication

Study funded by

not stated/no funding

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