To study whether a low frequency homogeneous magnetic field produces alterations of anti-cancer drugs potency in order to establish the basis for possible chemotherapy-sensitization by magnetic fields.
The following anti-cancer drugs were tested in yeast (Saccharomyces cerevisiae): cisplatin, mitomycin C and methotrexate (100 and 1000 µg/ml). Different experimental conditions were studied: 1) magnetic field exposure, 2) drug exposure, 3) magnetic field and drug exposure, and 4) un-exposed control group. Additionally, to assess the effects of temperature on cisplatin (1000 µg/ml), yeast cells were exposed to 20 °C, 27 °C and 30 °C.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for 48 h
|
|
cells were treated in four groups: i) exposed to MF ii) drug exposed iii) MF + drug exposed iv) unexposed control
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 48 h |
| Exposure source | |
|---|---|
| Setup | pair of Helmholtz coils with a diameter of 40 cm, 20 cm apart, mounted on a wooden frame, connected in series; each coil consisting of 154 turn of 1.4 mm copper wire |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 2.45 mT | - | measured | - | - |
Magnetic field exposure did not induce alterations in the potency of cisplatin, mitomycin C and methotrexate (at the doses assayed) on yeast cells in comparison to untreated control groups. There was no difference between magnetic field exposed groups and un-exposed control groups. A strong positive correlation between temperature and potency of cisplatin was found (importance of temperature control in experiments with anti-neoplastic agents in co-exposure with magnetic fields).
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