The effects of six cytostatics on the cell viability of Escherichia coli with and without exposure to a 60 Hz magnetic field should be investigated. Additionally, the influence of different field frequencies should be tested in this respect.
Each experiment was replicated six or seven times. The drugs tested were mitoxantrone, daunomycin, actinomycin D, bleomycin, zinostatin and mitomycin C. These were chosen because of their different molecular masses and modes of action.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
60 Hz
Exposure duration:
continuous for 6 h
|
|
|
Exposure 2:
20–280 Hz
Exposure duration:
continuous for 6 h
|
|
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 6 h |
| Exposure source |
|
|---|---|
| Setup | the temperature within the experimental area was controlled by circulating water at 36°C |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 50 mT | - | measured | - | - |
| Frequency | 20–280 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 6 h |
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 50 mT | - | measured | - | only in test with mitomycin C |
In the experiments with mitoxantrone, daunomycin, actinomycin D and bleomycin, a distinct and significant decrease of viable cells could be observed in the approaches with exposure to the magnetic field and to the drug compared to approaches without the magnetic field. This was interpreted as an enhancement of the effects of the drugs with enhancements of ca. 30%, depending on the drug. The approaches with zinostatin, however, showed only an enhancement of the effects of 3% when exposed to the magnetic field simultaneously. The experiments on the influence of the field frequency on the effects of mitomycin C showed the strongest effect at 60 Hz.
The authors conclude that 60 Hz magnetic fields increase the cytostatic effects of the examined drugs on E. coli and that the magnitude of this increase depends on the frequency of the field and the molecular mass of the drug.
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