To study whether the effects of extremely low frequency pulsed electromagnetic fields on cell proliferation, cell cycle and cell differentiation are similar in different-derived osteoblast-like cells (primary cell culture and cell line).
The effects of magnetic fields on morphology, proliferation, differentiation and mineralization of different kinds of osteoblast cells are studied to elucidate the mechanisms of osteogenesis.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
48 Hz
Modulation type:
pulsed
Exposure duration:
continuous for 24 h or 48 h
|
|
cells were treated in four ways: i) cells cultured for 24 h under PEMF ii) cells cultured for 24 h without PEMF, then exposed for another 24 h to PEMF iii) cells exposed to PEMF for 24 h, then cultured for another 24 h without PEMF iv) cells exposed to PEMF for 48 h
| Frequency | 48 Hz |
|---|---|
| Type | |
| Waveform |
|
| Exposure duration | continuous for 24 h or 48 h |
| Modulation type | pulsed |
|---|---|
| Pulse width | 13 ms |
| Repetition frequency | 48 Hz |
| Pulse type | monophasic |
| Exposure source | |
|---|---|
| Setup | 160 turns of 2.06 mm enamel copper wire wound around a 350 mm long cylindrical tube made of epoxy resins with an inner diameter of 190 mm, so that two sub-coils were created; cells placed in the center of the solenoid that was inside an incubator |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1.55 mT | - | measured | - | - |
1) MC3T3-E1 cells: Exposure to extremely low frequency pulsed electromagnetic field did not affect the cell number. The cell percentage of S phase and G2 phase decreased significantly after 24 h and 48 h. No alkaline phosphatase activity was detected.
2) Primary osteoblast cells: The cell number did not alter after 24 h continuous exposure, but increased greatly after 48 h. The cell percentage of S phase and G2 phase increased significantly after 24 h and decreased significantly after 48 h. The alkaline phosphatase activity decreased significantly after 24 h exposure and increased significantly after 48 h exposure.
In conclusion, in contrast to the MC3T3-E1 cell line, the primary osteoblast cells seemed to be more sensitive to the extremely low frequency-pulsed electromagnetic field.
This website uses cookies to provide you the best browsing experience. By continuing to use this website you accept our use of cookies.
