To study the ability of microorganisms to activate strategies in adapting themselves to the environmental stress induced by exposure to extremely low frequency electromagnetic fields, cultures of Escherichia coli were exposed at 50 Hz (0.1, 0.5, 1.0 mT).
Culture samples were exposed for 20 min. (acute exposure) or 120 min (about 6 generations, chronic exposure) and replaced outside of the switched off solenoid in the incubator for 24 h with an electromagnetic field background of 100 nT (50 Hz). Five independent experiments were performed and each experiment in triplicate.
Exposure | Parameters |
---|---|
Exposure 1:
50 Hz
Exposure duration:
continuous for 20 or 120 min
|
|
Frequency | 50 Hz |
---|---|
Type | |
Waveform | |
Exposure duration | continuous for 20 or 120 min |
Exposure source | |
---|---|
Setup | cells in glass flasks located on a non-magnetic support inside the coil in the maximum homogenity part of the magnetic field |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
magnetic flux density | 0.1 mT | minimum | measured | - | +/- 2 % |
magnetic flux density | 0.5 mT | - | measured | - | +/- 2 % |
magnetic flux density | 1 mT | maximum | measured | - | +/- 2 % |
Exposed and sham exposed samples showed similar cell counts, whereas an increased cell viability was observed in exposed samples re-incubated for 24 h outside of the solenoid compared to the corresponding controls.
An exposure of 20-120 minutes produced a significant change of morphotype with a presence of coccoid cells also aggregated in clusters after re-incubation of 24 h outside of the solenoid. Atypical lengthened bacterial forms were also found.
No changes among genotypical and transcriptional profiles were found for each electromagnetic field intensities evaluated.
The data indicate that an exposure to 50 Hz electromagnetic field acts as a stress factor on bacteria which can represent a suitable model to study acute and chronic effects related to extremely low frequency electromagnetic field exposure.
This website uses cookies to provide you the best browsing experience. By continuing to use this website you accept our use of cookies.