Study type: Medical/biological study (experimental study)

Effects of a static magnetic field on cell growth and gene expression in Escherichia coli med./bio.

By: Potenza L, Ubaldi L, De Sanctis R, De Bellis R, Cucchiarini L, Dacha M
Published in: Mutat Res Genet Toxicol Environ Mutagen 2004; 561 (1-2): 53-62
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Aim of study (acc. to author)

To investigate the effect of a static magnetic field (SMF) on cell growth and gene expression in Escherichia coli.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: up to 50 h

Exposure 1

Main characteristics
Frequency
Type
Exposure duration up to 50 h
Exposure setup
Exposure source
Chamber Shaker incubator maintained at 37°C, 120 strokes per min and shielded with Faraday cage.
Setup The magnets were inserted into slits carved on a polystyrene plate and placed underneath the 50 ml cell culture flasks with the magnetic field vertically oriented
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 300 mT maximum measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No change of cell growth could be found at cells cultured in LB-media. However, use of modified LB-media (containing glutamic acid and sodium chloride) leads to a significant increased growth of exposed cells.
Investigation of gene expression revealed altered expression patterns between exposed and no-exposed cells.

Study character:

Study funded by

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