Study type: Medical/biological study (experimental study)

Effect of high-frequency electromagnetic fields with a wide range of SARs on chromosomal aberrations in murine m5S cells med./bio.

By: Komatsubara Y, Hirose H, Sakurai T, Koyama S, Suzuki Y, Taki M, Miyakoshi J
Published in: Mutat Res Genet Toxicol Environ Mutagen 2005; 587 (1-2): 114-119
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Aim of study (acc. to author)

To study the induction of chromosomal aberrations in mouse cells after exposure to high frequency electromagnetic fields at 2.45 GHz with a wide range of SAR-values.

Background/further details

Mitomycin C (0.1 µg/ml) and x-ray (3 Gy) treatment were used as positive controls.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: continuous for 2 h
  • SAR: 100 W/kg maximum (50 W/kg, 20 W/kg, 10 W/kg and 5 W/kg)
Exposure 2: 2.45 GHz
Modulation type: pulsed
Exposure duration: intermittent for 2 h, 1 s field on/8 or 17 s off
  • SAR: 50 W/kg mean (900 W/kg corresponding peak value applied for 1 s followed by 17 s pause resulting in 50 W/kg mean value)
  • SAR: 100 W/kg mean (900 W/kg corresponding peak value applied for 1 s followed by 8 s pause resulting in 100 W/kg mean value)

Exposure 1

Main characteristics
Frequency 2.45 GHz
Charakteristic
  • guided field
Exposure duration continuous for 2 h
Additional info Reference article: S.Koyama, Y.Isozumi, Y.Suzuki, M.Taki, J.Miyakoshi. Effects of 2.45 GHz electromagnetic fields with a wide range of SARs on micronucleus formation in CHO-K1 cells, Scientific World Journal 4 (2004) 29-40.
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber One end of the waveguide was terminated with short circuit plate to generate standing waves.
Setup Sham exposure was conducted in the same manner as the RF exposure but in the absence of RF field. Positive control cells were treated with 3 Gy of X-ray or mitomycin C (0.1 µg/ml) for 2 h.
Parameters
Measurand Value Type Method Mass Remarks
SAR 100 W/kg maximum unspecified - 50 W/kg, 20 W/kg, 10 W/kg and 5 W/kg

Exposure 2

Main characteristics
Frequency 2.45 GHz
Charakteristic
  • guided field
Exposure duration intermittent for 2 h, 1 s field on/8 or 17 s off
Modulation
Modulation type pulsed
Pulse width 1 s
Additional info

30 s cycle
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 50 W/kg mean unspecified - 900 W/kg corresponding peak value applied for 1 s followed by 17 s pause resulting in 50 W/kg mean value
SAR 100 W/kg mean unspecified - 900 W/kg corresponding peak value applied for 1 s followed by 8 s pause resulting in 100 W/kg mean value

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant differences were revealed following exposure to high frequency electromagnetic fields at SAR values from 5 to 100 W/kg (continuous wave) and at a mean SAR of 100 W/kg (pulsed wave, a maximum SAR of 900 W/kg) compared with sham-exposed controls.
Treatments with mitomycin C and X-rays increased the frequency of chromatid-type and chromosome-type aberrations.
In conclusion, high frequency electromagnetic field exposures at 2.45 GHz for two hours with up to 100 W/kg SAR (continuous wave) and an average SAR of 100 W/kg (pulsed wave, a maximum SAR of 900 W/kg) do not induce chromosome aberrations in these mouse cells. Furthermore, there was no difference between exposures to continuous wave and pulsed wave high frequency electromagnetic fields.

Study character:

Study funded by

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