To study morphological modifications, i.e. cell shape, cell surface sugar residues, cytoskeleton, and apoptosis of Hep G2 cells (a hepatic transformed cell line) during 24 h exposure to 6 mT static magnetic field.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
Exposure duration:
continuous for 24 h
|
|
| Frequency | |
|---|---|
| Type | |
| Exposure duration | continuous for 24 h |
| Additional info | Static magnetic field |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 6 mT | - | measured | - | - |
The results indicate that 6 mT static magnetic field exposure exerts time dependent biological effects on Hep G2 cells.
Progressive modifications of cell shape and surface were found during the entire period of exposure. Exposed cells were elongated with many irregular microvilli randomly distributed on the cell surface.
At the end of the irradiation period, the cells had a less flat shape due to partial detachment from the culture dishes. However, throughout the period of irradiation, the morphology of the organelles remained unmodified and cell proliferation was only partially affected. In parallel with cell shape changes, the microfilaments and microtubules, as well as the quantity and distribution of surface Ricinus communnis-FITC and ConA-FITC labeling sites, were modified in a time dependant manner.
Apoptosis, which was almost negligible at the beginning of experiment, increased to about 20% after 24 h of continuous exposure. The induction of apoptosis was likely due to the increment of intracellular calcium concentration during exposure.
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