Study type: Medical/biological study (experimental study)

Synergistic effect of microwave heating and hydrogen peroxide on inactivation of microorganisms med./bio.

By: Kuchma T
Published in: J Microw Power Electromagn Energy 1998; 33 (2): 77-87
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Aim of study (acc. to author)

To study the combined effects of microwave heating with hydrogen peroxide simultaneously on cellular (bacterial survival) and molecular levels.

Endpoint

Exposure

Exposure
Exposure 1: 2.375 GHz
Exposure duration: until temperatures of 40°C , 50°C and 60°C

Exposure 1

Main characteristics
Frequency 2.375 GHz
Charakteristic
  • guided field
Exposure duration until temperatures of 40°C , 50°C and 60°C
Exposure setup
Exposure source
Setup Portions of cell suspension were added to the cylindrical test tubes (18 mm diameter) with cone shape ends
Additional info The sample containers were located in the centre of the standard rectangular waveguide parallel to the electric field
Parameters

No parameters are specified for this exposure.

Additional parameter details

Absorbed power density was 25,2 W/cm³ for time periods 3, 5 and 7 seconds

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The synergistic effect of microwave heating and low concentrations of hydrogen peroxide reached a maximum when the cells were exposed to microwave heating to 50°C and 0.08 % hydrogen peroxide simultaneously. Both maxima of cell destruction and DNA injuries have been achieved by successive exposure (microwaves + 10 min hydrogen peroxide) to 60°C and 0.08% hydrogen peroxide.

Study character:

Study funded by