Study type: Medical/biological study (experimental study)

Electromagnetic fields increase in vitro and in vivo angiogenesis through endothelial release of FGF-2 med./bio.

By: Tepper OM, Callaghan MJ, Chang EI, Galiano RD, Bhatt KA, Baharestani S, Gan J, Simon B, Hopper RA, Levine JP, Gurtner GC
Published in: FASEB J 2004; 18 (11): 1231-1233
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Aim of study (acc. to author)

To investigate the effects of pulsed electromagnetic fields (PEMF) on angiogenesis in vitro and in vivo.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 15 Hz
Modulation type: pulsed
Exposure duration: continuous for 24 h.
Exposure 2: 15 Hz
Modulation type: pulsed
Exposure duration: repeated daily exposure, 8 h/day for 14 days.

General information

In vitro expeiment.

Exposure 1

Main characteristics
Frequency 15 Hz
Type
Exposure duration continuous for 24 h.
Modulation
Modulation type pulsed
Pulse width 4.5 ms
Additional info

Asymmetric pulses.
Exposure setup
Exposure source
  • bone healing device.
Chamber incubator
Setup PEMF generators were kept in the incubator.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.2 mT maximum - - -
Additional parameter details

Magnetic flux density rising from 0 to 1.2 mT in 200 µs and returning to 0 mT in 25 µs.

Exposure 2

Main characteristics
Frequency 15 Hz
Type
Exposure duration repeated daily exposure, 8 h/day for 14 days.
Modulation
Modulation type pulsed
Pulse width 4.5 ms
Additional info

Asymmetric pulses.
Exposure setup
Exposure source
  • bone healing device.
Setup Mice were injected with matrigel and housed in cages where they were exposed.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.2 mT maximum - - -
Additional parameter details

Magnetic flux density rising from 0 to 1.2 mT in 200 µs and returning to 0 mT in 25 µs.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure with pulsed electromagnetic fields increased proliferation and tubulization of endothelium cells in vitro. Proliferation and migration of endothelium cells and fibroblasts could be increased by treatment with media of former PEMF treated cells. No effects on osteoblast could be found. Espacially FGF-2 (i.e. fibroblast growth factor) was found to be increased.
In vivo PEMF exposure increased angiogenesis more than twofold.

Study character:

Study funded by

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