To verify whether extremely low frequency magnetic fields could affect transposition activity like some environmental stress factors such as heat shock or UV irradiation.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
experiment 1: continuous for 34 h; experiment 2: continuous for 58 h
|
|
The magnetic fields and the frequency used in the study were selected for the exposure as they are a common exposure in urban environments and in domestic appliances.
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | experiment 1: continuous for 34 h; experiment 2: continuous for 58 h |
| Exposure source | |
|---|---|
| Chamber | incubator at 37°C |
| Setup | five Petri dishes (9 cm in diameter) were placed in the center of the coil system |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 100 µT | effective value | measured | - | - |
| magnetic flux density | 0.2 mT | - | - | - | - |
| magnetic flux density | 0.5 mT | - | - | - | - |
| magnetic flux density | 1 mT | - | - | - | - |
Bacteria that had been exposed for a long time (58 h) to a 50 Hz low intensity magnetic field (0.1-1 mT) gave colonies with significantly lower transposition activity compared to sham-exposed bacteria. Such reduction in transposition activity was positively correlated to the intensity of the magnetic field, in a dose-effect manner. This phenomenon was not affected by bacterial cell proliferation, since no significant differences were found in number, diameter and perimeter between exposed and sham-exposed colonies.
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