To determine if microwave exposure could elicit a biological effect in the absence of thermal stress, chick embryos were exposed to athermal microwave radiation (915 MHz) to look for induction of Hsp70 and oxidative stress protection.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
915 MHz
Exposure duration:
continuous for 30 min
|
|
|
Exposure 2:
30–90 Hz
Exposure duration:
continuous for 30 min
|
|
| Frequency | 915 MHz |
|---|---|
| Type | |
| Charakteristic |
|
| Exposure duration | continuous for 30 min |
| Exposure source | |
|---|---|
| Chamber | The Crawford cell having two chambers was mounted vertically on a table in a water-jacketed incubator maintained at 37.8°C. |
| Setup | Four eggs were placed vertically in each chamber with their narrow side down using holders on Plexiglas shelves. The MW signal was routed from top to bottom. Control eggs were placed in the same holders outside of the cell but in the same incubator. Matching of the Crawford cell was performed by adjusting the double stub tuner prior to each exposure period using dummy egg samples. |
| Additional info | Temperature increase in the samples was ~1.5°C at 5 W. Positive control was performed by placing eggs in a water-tight bag in a 43°C water bath for 1 h. |
| Frequency | 30–90 Hz |
|---|---|
| Type | |
| Exposure duration | continuous for 30 min |
| Modulation type | cf. additional info |
|---|---|
| Additional info |
random noise |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 20 µT | - | - | - | - |
Microwave frequency electromagnetic fields stress embryonic cells and, without causing a significant temperature increase, enhance Hsp70 protein levels. Chick embryos pre-exposed to radiofrequency electromagnetic fields prior to hypoxic stress had significantly higher survival rates after the hypoxic insult than controls.
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