Study type: Medical/biological study (experimental study)

[Growth behaviour of human leukemia cells] med./bio.

Wachstumsverhalten von humanen Leukämiezellen

By: Fitzner R, Langer E, Zemann E
Published in: Edition Wissenschaft 1995; 1: 3-12
Journal not peer-reviewed
Download citation in RIS format

Aim of study (acc. to author)

To investigate the effects of high frequency electromagnetic fields on growth behaviour of human leukemia cells in vitro. Furthermore the specific release (i.e appearence in supernatant) of the enzyme thymidine kinase was investigated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: pulsed
Exposure duration: continuous for 24 h
Exposure 2: 1.8 GHz
Modulation type: pulsed
Exposure duration: continuous for 24 h and 8 h

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 24 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Exposure setup
Exposure source
Chamber A constant temperature of 37 °C ± 0.1 °C in the experiment was maintained by an oil thermostat system.
Setup Test tubes with cell suspensions were placed in a Plexiglas sample holder connected to the oil thermostat and were positioned at an angle of 45° by means of a Styrofoam holder.
Sham exposure A sham exposure was conducted.
Additional info An identical sample holder was used to position the control cells inside a shielding RF-box.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 19 V/m - calculated - -
magnetic field strength 0.5 A/m - calculated - -
magnetic flux density 0.6 µT - calculated - -
SAR 80 mW/kg cf. remarks calculated - target value
Additional parameter details

Values indicated refer to vertical sample position inside the GTEM cell, the actual values with the position at 45° being lower.

Measurement and calculation details

The field values inside the culture medium could only be calculated with some simplifications. Hence they can deviate from the real values by a factor of two above or below. The SAR values aimed at could only be roughly achieved, since due to time constraints the calculations were made while the biological experiments were already running. Most of the calculated values are below these standard values.

Exposure 2

Main characteristics
Frequency 1.8 GHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 24 h and 8 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 26 V/m - calculated - -
magnetic field strength 0.8 A/m - calculated - -
magnetic flux density 1 µT - calculated - -
SAR 80 mW/kg cf. remarks calculated - target value

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Doubling time and thymidine kinase release were not significantly altered due to electromagnetic field exposure. Therefore a possible cancerogenic effect of electromagnetic fields as used in this study could not be determined.

Study character:

Study funded by

Related articles