Study type: Medical/biological study (experimental study)

The relationship between colony-forming ability, chromosome aberrations and incidence of micronuclei in V79 Chinese hamster cells exposed to microwave radiation med./bio.

By: Garaj-Vrhovac V, Horvat D, Koren Z
Published in: Mutation Research - Letters 1991; 263 (3): 143-149
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Aim of study (acc. to author)

To study the effect of microwave radiation on cell survival and on the incidence and frequency of micronuclei and structural chromosome aberrations.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 7.7 GHz
Exposure duration: 10, 20, 30 and 60 min

Exposure 1

Main characteristics
Frequency 7.7 GHz
Type
Waveform
Exposure duration 10, 20, 30 and 60 min
Exposure setup
Exposure source
Setup samples placed below the horn antenna on a thin bakelite plate (1.2 m high).
Additional info for more details on exposure set-up pls see reference article.
Parameters
Measurand Value Type Method Mass Remarks
power density 5 W/m² - cf. remarks - 10 mW/cm² and 30 mW/cm²; culture irradiated in a logarithmical phase of growth
power 2 W - cf. remarks - max output of the generator
Additional parameter details

After two days of exponential growth the culture was irradiated in a semi permeable membrane with a power density of 30 mW/cm² for 15, 30 and 60 min.

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The results indicate that microwave radiation can induce damage in the structure of chromosomal DNA: There was a significantly higher frequency of specific chromosome aberrations such as dicentric and ring chromosomes in irradiated cells in comparison to the control samples. The presence of micronuclei in exposed cells confirmed the changes that had occured in chromosome structure. The decrease in the number of exposed cell colonies was related to the power density applied and to the time of irradiation.

Study character:

Study funded by

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