Study type: Medical/biological study (experimental study)

Altered restriction patterns of microwave irradiated lambda-phage DNA med./bio.

By: Narasimhan V, Huh WK
Published in: Biochem Int 1991; 25 (2): 363-370
Journal partially peer-reviewed/peer-review process unclear
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Aim of study (acc. to author)

To study the influence of microwave irradiation on the conformation of λ phage (lambda phage) DNA. The DNA samples were subjected to restriction fragmentation by EcoRI and BamHI (i.e. the restriction enzymes cut/digest the DNA and produce fragments of different lengths).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Exposure duration: 2, 4, 8, 12, 16, and 20 sec
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Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Waveform
Exposure duration 2, 4, 8, 12, 16, and 20 sec
Exposure setup
Exposure source
Setup 100 µl aliquots were placed in stoppered microcentrifuge tubes and suspended in a beaker of ice cold water (5° to 8°C). The beaker containing the samples was placed in the mirowave oven.
Parameters

No parameters are specified for this exposure.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

EcoRI fragmentations of microwaved DNA samples yielded three additional fragments, while BamHI fragmentations did not yield any additional fragments. Mobilities of the BamHI fragments from the microwaved DNA samples were slower and the bands were broader in comparison to those from native samples. These altered restriction patterns were attributed to the anomalies of conformation in DNA resulting from single strand breaks and localized strand separations induced by microwave exposure.

Study character:

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