Study type: Medical/biological study (experimental study)

Assessment of the effects of exposure to extremely low-frequency magnetic fields on MDCK epithelial cell lines under a controlled environment med./bio.

By: Domínguez G, Cardiel E, Sánchez E, Hernández PR
Published in: J Radiat Res 2021; 62 (2): 259-268
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Aim of study (acc. to author)

The effects of exposure of MDCK epithelial cells to 60 Hz magnetic fields on different cellular parameters should be investigated.

Background/further details

MDCK cells were divided into the following groups: 1) exposure to a 1 mT magnetic field (only used for transepithelial electrical impedance test), 2) exposure to a 5 mT magnetic field. For each exposure group, a separate control group was used.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 60 min every 4 h, over 24 h (only for ZO-1 measurement: 24 h exposure, 24 h no exposure, 24 h exposure)
Exposure 2: 60 Hz
Exposure duration: 60 min every 4 h, over 24 h (only for ZO-1 measurement: 24 h exposure, 24 h no exposure, 24 h exposure)

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 60 min every 4 h, over 24 h (only for ZO-1 measurement: 24 h exposure, 24 h no exposure, 24 h exposure)
Exposure setup
Exposure source
Chamber all handling processes were performed in a controlled environment inside a chamber, where temperature, CO2 and humidity were regulated
Setup a uniform magnetic field was produced and a maximum temperature increase of 0.4 °C was measured after 12 h exposure to the 5 mT magnetic field
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT peak-to-peak value measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 60 min every 4 h, over 24 h (only for ZO-1 measurement: 24 h exposure, 24 h no exposure, 24 h exposure)
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT peak-to-peak value measured - -

Reference articles

  • Domínguez G et al. (2017): Development of a Magnetic-field Stimulation System for Cell Cultures in situ: Simulation by Finite Element Analysis

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:

Main outcome of study (acc. to author)

The protein expression of CLDN-1 was decreased by 90% in the exposure group (group 2) compared with the control, while ZO-1 protein expression increased by 120%. No significant differences were observed in size and shape of the cell nuclei but a significant decline was observed in the density of cell nuclei in exposed cells compared to the control group. There were no significant differences in cell migration and cell proliferation.
The authors concluded that exposure to a 60 Hz magnetic field might have effects on MDCK epithelial cells.

Study character:

Study funded by

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