The effects of exposure of mouse ovarian follicles to a 33 Hz or 50 Hz magnetic field on the follicle development should be investigated.
Pre-antral follicles were isolated from 22-day old mice and were either exposed to a 33 Hz magnetic field (group 1), a 50 Hz magnetic field (group 2) or not exposed (control group) for 5 days.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
33 Hz
Modulation type:
pulsed
Exposure duration:
continuous for 5 days
|
|
|
Exposure 2:
50 Hz
Modulation type:
pulsed
Exposure duration:
continuous for 5 days
|
|
| Modulation type | pulsed |
|---|---|
| Duty cycle | 50 % |
| Exposure source | |
|---|---|
| Chamber | plates with 96 wells (one follicle/well) |
| Setup | field was homogeneous over 64 wells of a 96-well plate; plate was placed in central region of the coil (no contact); temperature was maintained at 37 ± 0.1°C inside each well, CO2 concentration was 5% |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1.5 mT | - | measured | - | ± 0.01 mT |
| Modulation type | pulsed |
|---|---|
| Duty cycle | 50 % |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1.5 mT | - | measured | - | ± 0.01 mT |
Growth was significantly reduced in 33 Hz-exposed follicles (group 1), but not in 50 Hz-exposed follicles (group 2), after 5 days compared to the control group. In both exposure groups, antrum formation was significantly impaired compared to the control group and follicles with failed antrum formation showed a significantly reduced concentration of estradiol in the culture medium (remark EMF-Portal: results for group 2 were not described distinctly). Cell proliferation was significantly reduced in comparison to the control group as well. Apoptosis was not significantly altered between the groups.
The authors conclude that exposure of mouse ovarian follicles to a 33 Hz or 50 Hz magnetic field might have detrimental effects on the follicle development.
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