The effects of chronic exposure of orange-spotted cockroach nymphs to a static magnetic field or 50 Hz magnetic field on gut mass and antioxidant markers should be investigated.
Nymphs of orange-spotted cockroach (Blaptica dubia) were divided into the following groups at the age of one month (n=10, respectively): 1) exposure to a static magnetic field, 2) exposure to a 50 Hz magnetic field, 3) control group.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
Exposure duration:
for 5 months
|
|
|
Exposure 2:
50 Hz
Exposure duration:
for 5 months
|
|
| Frequency | |
|---|---|
| Type | |
| Exposure duration | for 5 months |
| Exposure source | |
|---|---|
| Chamber | 3-5 nymphs in Petri dishes |
| Setup |
a double-U shaped permanent magnet; Petri dishes were placed between the poles (distance 4.5 cm); the magnetic field lines were parallel to the vertical component of the geomagnetic field; the temperature was stable during the experiment with 26 ± 0.2°C |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 110 mT | - | measured | - | field homogeneity ± 26.87% |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | for 5 months |
| Exposure source |
|
|---|---|
| Chamber | see field 1 |
| Setup |
electromagnet consisting of a regular laminated transformer core surrounded by three pairs of coils; each coil was made of 84 copper wires (1 mm in diameter); the surface of the poles was 44.6 cm2 with a 7.5 cm space between them; the magnetic field lines were parallel to the vertical component of the geomagnetic field; the average temperature was 26.3 ± 0.05°C |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 10 mT | mean | measured | - | approximate value with uniformity of ± 69.12% |
The average weight of the gut was significantly reduced in exposed nymphs (groups 1 and 2) compared to the control group. The enzyme activities of superoxide dismutase and catalase were significantly increased in exposed nymphs of groups 1 and 2 compared to the control group, while the enzyme activities of glutathione reductase and glutathione S-transferase were significantly reduced. In addition, the enzyme activities of superoxide dismutase and glutathione reductase in group 2 (exposure to 50 Hz magnetic field) were significantly increased compared to group 1 (exposure to static magnetic field). The total glutathione content showed no significant differences between the groups.
The authors conclude that chronic exposure of orange-spotted cockroach nymphs to a static magnetic field or 50 Hz magnetic field could alter the weight of the gut and the investigated antioxidative markers.
This website uses cookies to provide you the best browsing experience. By continuing to use this website you accept our use of cookies.
