Study type: Medical/biological study (experimental study)

Eotaxin‑1 and MCP‑1 serve as circulating indicators in response to power frequency electromagnetic field exposure in mice med./bio.

Published in: Mol Med Rep 2018; 18 (3): 2832-2840

Aim of study (acc. to author)

To examine the level of chemokines in the blood of mice exposed to 50 Hz magnetic fields.

Background/further details

Mice were divided into four groups (n=100 per group): 1) sham exposure, 2) 0.1 mT magnetic field, 3) 0.5 mT magnetic field and 4) 2.5 mT magnetic field. Mice were exposed/sham exposed for 0, 1, 10, 30, or 90 consecutive days. At every time point, 20 mice of very group were anesthetized for blood sampling and afterwards sacrified.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 8 hours/day for 0, 1, 10, or 90 consecutive days
Exposure 2: 50 Hz
Exposure duration: 8 hours/day for 0, 1, 10, or 90 consecutive days
Exposure 3: 50 Hz
Exposure duration: 8 hours/day for 0, 1, 10, or 90 consecutive days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 8 hours/day for 0, 1, 10, or 90 consecutive days
Exposure setup
Exposure source
Chamber mice were housed in standardized cages (10 mice per cage); all cages were placed in the same room
Setup a transformer was used to provide electric current to the coils for generating the magnetic field; the homogenous region of the magnetic field was 0.4 x 0.4 x 0.4 (m) with harmonics below 1.0 %; magnetic field inside the cage was kept constant; magnetic field was switched on at least one minute before exposure to avoid transients
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT effective value measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration 8 hours/day for 0, 1, 10, or 90 consecutive days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT effective value measured - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Exposure duration 8 hours/day for 0, 1, 10, or 90 consecutive days
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2.5 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

Exposure to different magnetic flux densities caused significant alterations in the levels of the following chemokines: IP‑10, GROα, RANTES, EOTAXIN‑1, and MCP‑1 (Remark EMF-Portal: stated in running text, partially not comprehensive from tables). Moreover, both the immunoassay and the ELISA showed that the levels of MCP-1 and EOTAXIN-1 were significantly increased in mice exposed to the 0.5 mT magnetic field compared to the control group.
(Remark EMF-Portal: Chemokine concentrations on day zero were the same in every dose group which indicates that the authors did not examine the samples of the single groups.)
The authors conclude that EOTAXIN-1 and MCP-1 could be used as indicators for 50 Hz-magnetic field exposure and suggest that 50 Hz magnetic field could induce inflammation responses in mice.

Study character:

Study funded by

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