Medical/biological study (experimental study)

Cell oxidation-reduction imbalance after modulated radiofrequency radiation med./bio.

By: Marjanovic AM, Pavicic I, Trosic I

Published in: Electromagn Biol Med 2015; 34 (4): 381-386

Aim of study (acc. to author)

The effects of exposure of hamster fibroblasts to a 1800 MHz electromagnetic field on cell viability and oxidative stress should be investigated.

Background/further details

Cells were either exposed to the electromagnetic field or not exposed (control group) for 10, 30 or 60 minutes. A positive control was conducted and all data were obtained from triplicate measurements.

Endpoint

Exposure

- 1,800 MHz
- mobile communications
- mobile phone
- GSM

Exposure	Parameters
Exposure 1: 1,800 MHz Exposure duration: continuous for 10, 30 and 60 minutes	electric field strength: 30 V/m SAR: 1.6 W/kg

Exposure 1

Main characteristics

Frequency	1,800 MHz
Type	electromagnetic field
Exposure duration	continuous for 10, 30 and 60 minutes

Modulation

Modulation type	cf. additional info
Additional info	GSM-modulation

Exposure setup

Exposure source	GTEM cell
Setup	GTEM cell was equipped with a signal generator, signal amplifier and a signal modulator; temperature was measured and preserved at a physiological level

Parameters

Measurand	Value	Type	Method	Mass	Remarks
electric field strength	30 V/m	-	-	-	-
SAR	1.6 W/kg	-	calculated	-	-

Exposed system:

intact cell/cell culture
V79 (Chinese hamster fibroblast cells)

Methods Endpoint/measurement parameters/methodology

molecular biosynthesis: total protein content (Bradford protein assay)
cell viability/cell division/proliferation: cell viability (MTT assay)
oxidative stress: content of carbonyl proteins (ELISA) and intracellular reactive oxygen species (oxidation of dichlorofluorescein, spectrophotometry)

Investigated system:

isolated bio./chem. substance
intact cell/cell culture

Time of investigation:

during exposure
after exposure

Main outcome of study (acc. to author)

The amount of reactive oxygen species was significantly increased after 10 minutes of exposure compared to the control group. After 30 and 60 minutes, however, no differences were observed. All other parameters did not show any significant differences between the exposure and control group.
The authors conclude that exposure of hamster fibroblasts to a 1800 MHz electromagnetic field might influence the oxidative state in cells.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Ministry of Science and Technology (later on "Ministry of Science, Education and Sport"), Croatia

Hou Q et al. (2015): Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3T3 cells
Kang KA et al. (2014): Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells
Ni S et al. (2013): Study of Oxidative Stress in Human Lens Epithelial Cells Exposed to 1.8 GHz Radiofrequency Fields
Hong MN et al. (2012): Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or combined on oxidative stress in MCF10A cells
Lu YS et al. (2012): Reactive Oxygen Species Formation and Apoptosis in Human Peripheral Blood Mononuclear Cell Induced by 900 MHz Mobile Phone Radiation
Brescia F et al. (2009): Reactive oxygen species formation is not enhanced by exposure to UMTS 1950 MHz radiation and co-exposure to ferrous ions in Jurkat cells
Yao K et al. (2008): Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells