A hemispheric plastic Millipore filter holder having a base diameter of 25 mm and a volume of 1 cm³ was mounted on a Plexiglas platform in the center of a Crawford cell with the filter 5 cm below the top of the chamber.
Setup
The filter was half-filled with temperature controlled solution at 31 °C delivered from a syringe at 1 ml/min throughout the perfusion. Perfusate-collecting vials were placed in a cardboard box with 6-mm walls on the septum and were moved manually one position every minute.
Additional info
Duplicate samples were prepared from the same incubation; one served as control and the other one was exposed to MW fields during perfusion. Both syringes were mounted on the same pump, and temperature was maintained by the same circulator. The effect of temperature alone was studied by perfusion at 25, 31, 33, and 35 °C. For stimulation by calcium, a concentrated CaCl2 solution was injected at a rate of 10µl/min for 5 min. Cholinechlorideinjection served as control.
Bureau of Radiological Health (later on "Center for Devices and Radiological Health" (CDRH) at FDA), USA
Department of Energy, USA
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