To determine if there are any differential effects produced by heating with microwave radiation compared to heating in a water bath on denaturation of DNA.
Exposure | Parameters |
---|---|
Exposure 1:
2.45 GHz
Modulation type:
CW
Exposure duration:
see additional information.
|
|
Frequency | 2.45 GHz |
---|---|
Type | |
Waveform | |
Exposure duration | see additional information. |
Modulation type | CW |
---|
Exposure source | |
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Setup | 30 ml plastic tissue culture flask mounted on a styrofoam support which was surrounded by large panels (7 ft high) of microwave absorber. |
Additional info | Exposurte time: a) continous for 16 hr at 37°C in a sodium citrate buffer, b) 16 hrs at 37°C in a solution containing formaldhyde, c) 1 hr at > 37°C in a solution containing formaldehyde. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
power density | 225 mW/cm² | - | calculated | - | at 50°C. |
power density | 940 W/m² | - | calculated | - | at 37°C. |
power density | 134 mW/cm² | maximum | measured | - | at 37°C. |
power density | 118 mW/cm² | - | calculated | - | at 40°C. |
power density | 158 mW/cm² | - | calculated | - | at 45°C. |
The experiments show that 2450 MHz (2.45 GHz) CW microwave irradiation under the exposure conditions employed has very little if any effect other than the effect of thermal heating on the secondary structure of DNA as determined by comparison of thermal denaturation curves.
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