Medical/biological study (experimental study)

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts med./bio.

By: Shckorbatov YG, Pasiuga VN, Goncharuk EI, Petrenko TP, Grabina VA, Kolchigin NN, Ivanchenko DD, Bykov VN, Dumin OM

Published in: J Zhejiang Univ Sci B 2010; 11 (10): 801-805

Aim of study (acc. to author)

To study the influence of microwave irradiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization (linear, left-handed and right-handed elliptically polarized) were examined.

Background/further details

The mean heterochromatin granule quantity was calculated for 30 nuclei and each experiment was repeated three times.

Endpoint

genotoxicity/mutation: chromatin condensation

Exposure

- 36.65 GHz
- microwaves
- millimeter waves

Exposure	Parameters
Exposure 1: 36.65 GHz Exposure duration: continuous for 10 s linear polarized field	power density: 1 µW/cm² (at the surface of the exposed object) power density: 10 µW/cm² (at the surface of the exposed object) power density: 30 µW/cm² (at the surface of the exposed object) power density: 100 µW/cm² (at the surface of the exposed object)
Exposure 2: 36.65 GHz Exposure duration: continuous for 10 s left-handed and right-handed elliptically polarized field	power density: 1 µW/cm² (at the surface of the exposed object) power density: 10 µW/cm² (at the surface of the exposed object) power density: 30 µW/cm² (at the surface of the exposed object) power density: 100 µW/cm² (at the surface of the exposed object)

Exposure 1

Main characteristics

Frequency	36.65 GHz
Type	electromagnetic field
Polarization	linear
Exposure duration	continuous for 10 s
Additional info	linear polarized field

Exposure setup

Exposure source	self-developed semi-conductor device
Setup	human fibroblasts growing in a monolayer on a cover slide, covered by 1 mm of culture medium during exposure
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
power density	1 µW/cm²	-	-	-	at the surface of the exposed object
power density	10 µW/cm²	-	-	-	at the surface of the exposed object
power density	30 µW/cm²	-	-	-	at the surface of the exposed object
power density	100 µW/cm²	-	-	-	at the surface of the exposed object

Exposure 2

Main characteristics

Frequency	36.65 GHz
Type	electromagnetic field
Polarization	elliptical
Exposure duration	continuous for 10 s
Additional info	left-handed and right-handed elliptically polarized field

Exposure setup

Exposure source	self-developed semi-conductor device + grating polarizer
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
power density	1 µW/cm²	-	-	-	at the surface of the exposed object
power density	10 µW/cm²	-	-	-	at the surface of the exposed object
power density	30 µW/cm²	-	-	-	at the surface of the exposed object
power density	100 µW/cm²	-	-	-	at the surface of the exposed object

Reference articles

Shckorbatov YG et al. (2009): The influence of differently polarised microwave radiation on chromatin in human cells

Exposed system:

intact cell/cell culture
primary human fibroblasts

Methods Endpoint/measurement parameters/methodology

genotoxicity/mutation: chromatin condensation: heterochromatin granule quantity (aceto-orcein stain; light microscopy, electron microscopy (heterochromatinization as marker for DNA damage))
cell viability/cell division/proliferation: cell viability (trypan blue exclusion assay)

Investigated system:

intact cell/cell culture

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Microwave irradiation at the intensity of 1 µW/cm² produced no effect, but exposure at the intensities of 10 and 100 µW/cm² induced an increase in heterochromatin granule quantity. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized irradiation revealed more biological activity than the left-handed polarized one.
As the left-handed elliptically polarized microwaves induced less heterochromatinization, they should be preferred for the use in various systems of radio communications.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

Shckorbatov YG et al. (2009): The influence of differently polarised microwave radiation on chromatin in human cells
Belyaev IY et al. (2005): 915 MHz microwaves and 50 Hz magnetic field affect chromatin conformation and 53BP1 foci in human lymphocytes from hypersensitive and healthy persons
Sarimov R et al. (2004): Nonthermal GSM Microwaves Affect Chromatin Conformation in Human Lymphocytes Similar to Heat Shock
Belyaev IY et al. (2000): Nonthermal effects of extremely high-frequency microwaves on chromatin conformation in cells in vitro - dependence on physical, physiological, and genetic factors
Shckorbatov YG et al. (1998): Microwave irradiation influences on the state of human cell nuclei

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

Exposure

Exposure 1

Main characteristics

Exposure setup

Parameters

Exposure 2

Main characteristics

Exposure setup

Parameters

Reference articles

Methods Endpoint/measurement parameters/methodology

Main outcome of study (acc. to author)

Study funded by

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