Medical/biological study (experimental study)

Cell Lysis in SWLA-2 Hybridomas due to 1 kHz AC Electric Fields med./bio.

By: Oakley B, Hanna D, Kandlikar S, Gross B, Stryker G

Published in: 2005 IEEE Engineering in Medicine and Biology 27th Annual Conference, Shanghai. IEEE, 2005: pp. 1268-1269; ISBN 978-0-7803-8741-6

The study was performed to gain an understanding of the parameters for the lysing of eukaryotic cells using a pulse of AC electric field.

AC pulses of varying pulse width were used.

Exposure	Parameters
Exposure 1: 1 kHz Modulation type: single pulse Exposure duration: single pulse with a width of 1 ms, 10 ms, 100ms, 500 ms or 1 s	power: 500 W

Frequency	1 kHz
Type	electric field
Waveform	sinusoidal
Exposure duration	single pulse with a width of 1 ms, 10 ms, 100ms, 500 ms or 1 s

Modulation type	single pulse

Exposure source	Eppendorf electroporation cuvettes with 1 mm gap
Sham exposure	A sham exposure was conducted.

Measurand	Value	Type	Method	Mass	Remarks
power	500 W	-	-	-	-

Exposed system:

Investigated system:

Time of investigation:

The data show different percentages of cell lysis of SWLA-2 murine hybridomas produced by AC electric field pulses at 1 kHz with pulse widths ranging from 1 ms to 1 second. However, heating may have contributed to cell lysis.

Study character:

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