Medical/biological study (experimental study)

Organ culture studies on the development of mouse embryo limb buds under EMF influence med./bio.

By: Parivar K, Kouchesfehani MH, Boojar MM, Hayati RN

Published in: Int J Radiat Biol 2006; 82 (7): 455-464

Aim of study (acc. to author)

To study the morphological and histological modifications induced by an electromagnetic field on the development of the limb bud in vitro.

Background/further details

20 limb buds per group (exposed, sham-exposed, control) were used.

Endpoint

morphol./histopathol. changes: development of limb buds in vitro

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 2 h	magnetic flux density: 13.1 mT unspecified

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuous for 2 h

Exposure setup

Exposure source	solenoid
Setup	The culture dishes were placed inside a plastic basket which was placed inside the coil mounted vertically in the incubator. The coils were turned off during the sham exposure.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	13.1 mT	unspecified	measured	-	-

Measurement and calculation details

MF was measured with a Gauss meter.

Exposed system:

isolated organ
organ culture system (limb buds)

Methods Endpoint/measurement parameters/methodology

cell viability/cell division/proliferation: cell proliferation and cell differentiation (number of red blood cells, mitotic cells, and chondrocytes; number of mesenchymal cells and degenerated cells)
morphol./histopathol. changes: development of limb buds in vitro (growth rate (length and/or width of limb bud, finger or toe, wrist, etc.); hematoxylin-eosin stain)

Investigated system:

intact cell/cell culture
tissue slices

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The growth rate in both fore and hindlimb buds (in proximal-distal and anterior-posterior axes) was significantly increased in exposed limb buds. Chondrocyte counts and mitotic figures of mesenchymal and red blood cells were significantly increased as compared with those of sham-exposed and control groups. There was also a significant reduction of mesenchymal cell counts, while no significant difference was found in the degenerated cell counts among the three groups.
These results suggest that electromagnetic fields, under the conditions applied, has progressive effects on the limb bud development and that both proliferation and cell differentiation can be stimulated in vitro. The authors suggest a chondrogenic potential for electromagnetic fields which may be useful for increasing the cell proliferation and regeneration of chondrogenic cells.

Study character:

medical/biological study
experimental study
full/main study
blind study

Study funded by

not stated/no funding

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