To investigate the chondrogenic effects (synthesis and turnover of cartilage matrix components) of electromagnetic fields, using organoid cultures of blastemal cells from limb buds of mouse embryos.
For simulating inflammatory conditions in vitro the cells were treated with bacterial lipopolysaccharides from day 4 onwards.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
150 MHz
Modulation type:
AM
Exposure duration:
continuous for 4 h
|
|
|
Exposure 2:
150 MHz
Modulation type:
AM
Exposure duration:
continuous for 24 h
|
|
|
Exposure 3:
150 MHz
Modulation type:
AM
Exposure duration:
repeated daily exposure, 4 h/day for 7 days
|
|
| Frequency | 150 MHz |
|---|---|
| Type | |
| Exposure duration | continuous for 4 h |
| Modulation type | AM |
|---|---|
| Modulation frequency | 16 Hz |
| Exposure source |
|
|---|---|
| Chamber | Electromagnetic chamber maintained at 37°C, 95% humidity and 5% CO2 |
| Setup | Culture dishes were placed in an electromagnetic exposure chamber. |
| Additional info | All cultures including the controls, which were placed in the same incubator without exposure to magnetic field, were treated with 10 mg/ml LPS (bacterial lipopolysaccharides) from day 4 of the culture. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| electric field strength | 4 mV/cm | mean | unspecified | - | - |
| magnetic flux density | 40 nT | unspecified | unspecified | - | - |
| Frequency | 150 MHz |
|---|---|
| Type | |
| Exposure duration | continuous for 24 h |
| Modulation type | AM |
|---|---|
| Modulation frequency | 16 Hz |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| electric field strength | 4 mV/cm | mean | estimated | - | - |
| magnetic flux density | 40 nT | unspecified | unspecified | - | - |
| Frequency | 150 MHz |
|---|---|
| Type | |
| Exposure duration | repeated daily exposure, 4 h/day for 7 days |
| Modulation type | AM |
|---|---|
| Modulation frequency | 16 Hz |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| electric field strength | 4 mV/cm | mean | unspecified | - | - |
| magnetic flux density | 40 nT | unspecified | unspecified | - | - |
High-frequency magnetic fields seem to cause a moderate enhancement of collagens and proteoglycans in spite of the indirect catabolic effects of lipopolysaccharides by enhancing the activity of metalloproteinases. The slight inhibitory effect of magnetic fields on metalloproteinases is too weak to reduce matrix degradation markedly, thus leading to accumulation of collagens. It is concluded that high-frequency magnetic fields exert anabolic effects on embryonal chondrogenic tissue in vitro.
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