To study the influence of static magnetic field on DNA binding activity and expression of nuclear AP-1 (activator protein-1) complex in immature and mature cultured rat cortical and hippocampal neurons as a guidepost for possible long-lasting functional alterations by magnetism in the brain.
c-Fos protein family is a partner of c-Jun protein family members to compose the nuclear transcription factor AP-1.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
Exposure duration:
continuous for 15 min
|
|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 100 mT | - | unspecified | - | - |
Brief exposure for 15 min to static magnetic field (100 mT) resulted in marked but transient expression of AP-1 complex with DNA binding activity in immature cultured hippocampal neurons. The data revealed that brief exposure to static magnetic field increased AP-1 DNA binding through expression of Fra-2, c-Jun, and Jun-D proteins in immature cultured hippocampal neurons.
Transient magnetic stimulation would induce long-lasting alterations of a variety of cellular functions through modulation of de novo synthesis of particular inducibel target proteins at the level of gene transcription by AP-1 complex in the nucleus of immature cultured hippocampal neurons. For example, prior brief exposure to magnetism could result in desensitization of NMDA (N-methyl-D-aspartate) receptor channels with regard to increase in intracellular free Ca2+ concentration (as determined by fluorescence imaging) and activator protein-1 binding in immature neurons.
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