To study whether in utero and neonatal exposure to a 60 Hz extremely low frequency electromagnetic field results in spermatotoxicity and reproductive dysfunction in the F1 offspring of rats.
Pregnant rats were exposed continuously (21h/day) to a 60 Hz extremely low frequency electromagnetic field from day 6 of gestation through to day 21 of lactation.
| Exposure | Parameters |
|---|---|
| Exposure 1: 60 Hz |
|
| Exposure source |
|
|---|---|
| Chamber | polycarbonate cages were placed on racks consisting of three levels (ten rats on each level). The spatial variation of the magnetic field was under 3% within the animal testing area (1 m x 1 m x 1 m). |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 500 mT | unspecified | measured | - | - |
| magnetic flux density | 83.3 mT | unspecified | measured | - | - |
| magnetic flux density | 5 mT | unspecified | measured | - | - |
No exposure-related alterations were revealed in exposed or sham-exposed rats with respect to the anogenital distance, preputial separation, testis weight, testicular histology, sperm count, daily sperm production, sperm motility, sperm morphology and reproductive capacity of F1 offspring.
The authors concluded that exposure of rats to a 60 Hz extremely low frequency electromagnetic field at field strengths of up to 500 mT from day 6 of gestation to day 21 of lactation did not produce any detectable alterations in offspring spermatogenesis and fertility.
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