Study type: Medical/biological study (experimental study)

Effects of mechanochemically activated doxorubicin and 40 MHz frequency irradiation on human A-549 lung carcinoma cells med./bio.

By: Orel VE, Kudryavets YI, Satz S, Bezdenezhnih NA, Danko MI, Khranovskaya NN, Romanov AV, Dzyatkovskaya NN, Burlaka AP, Sidorik EP
Published in: Exp Oncol 2004; 26 (4): 271-277
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Aim of study (acc. to author)

To study the effects of mechanochemically activated (MA) doxorubicin (DOXO) and exposure to electromagnetic fields (40 MHz) on human lung carcinoma A-549 cells.

Background/further details

The antibiotics doxorubicin was applied in two different forms: officinal doxorubicin (OF DOXO) and mechanochemically activated doxorubicin (MA DOXO). MA DOXO was yielded by mechanochemical activation applying friction and impact energy during grinding and consisted of particles with sizes 10 times smaller than OF DOXO.
Six groups were investigated: 1.) control group, 2.) EMF exposure, 3.) OF DOXO application, 4.) MA DOXO application, 5.) OF DOXO application + EMF exposure, and 6.) MA DOXO application + EMF exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 40 MHz
Exposure duration: continuous for 30 min

General information

Cells were treated in six groups: i) control - no treatment ii) EMF exposure iii) OF DOXO iv) MA DOXO v) OF DOXO + EMF exposure vi) MA DOXO + EMF exposure

Exposure 1

Main characteristics
Frequency 40 MHz
Type
Exposure duration continuous for 30 min
Exposure setup
Exposure source
  • not specified
Setup cells exposed in Petri dishes in a round irradiation frame with 3 cm diameter; temperature during exposure: 37°C
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 27.5 V/m - calculated - -
magnetic field strength 22 A/m - - - -
power density 2 W/cm² - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure to 40 MHz electromagnetic fields has a slight influence on the cell proliferation in comparison with the control cells without treatment. Cell survival decreased in the following way: EMF (group 2), OF DOXO application (group 3), MA DOXO application (group4), OF DOXO application + EMF (group 5), and MA DOXO application + EMF (group 6).
The authors concluded that the treatment by mechanochemically activated doxorubicin and drug with EMF showed better targeting of drug in human lung carcinoma A-549 cells outcomes than officinal doxorubicin.

Study character:

Study funded by

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