To test the hypothesis that DNA strand brakes induced by ELF-electromagnetic fields as observed in human diploid fibroblast cells meight be mediated by cellular changes of intracellular free calcium and/or mitochondrial membrane potential.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
intermittent 5 min on/10 min off for 15 hours or 7, 9, 11, 15, 17 hours
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | intermittent 5 min on/10 min off for 15 hours or 7, 9, 11, 15, 17 hours |
| Exposure source |
|
|---|---|
| Setup | two identical coil systems that were placed inside a commercial incubator to ensure constant environmental conditions (37°C, 5% CO2, 95% humidity) optimized to provide a homogeneous field at the area of the Petri dishes. Each coil system itself consisted of 4 subcoils (2 coils with 56 windings, 2 coils with 50 windings), which were placed inside a µ-metal box. |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | - | measured | - | - |
A significant increase of DNA strand brakes induced by ELF-EMF exposure was found. However, no increase of intracellular free calcium or changes in mitochondrial membrane potential could be detected. Therefore the results suggest that EMF-induced DNA strand breakes in human fibroblast are not mediated by the examined cellular processes in vitro.
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