Study type: Medical/biological study (experimental study)

Leukemic cell intracellular responses to nanosecond electric fields med./bio.

By: Chen N, Schoenbach KH, Kolb JF, Swanson RJ, Garner AL, Yang J, Joshi RP, Beebe SJ
Published in: Biochem Biophys Res Commun 2004; 317 (2): 421-427
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Aim of study (acc. to author)

To investigate the response of human HL-60 cells on nanosecond pulsed electric fields (nsPEFs) in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 17 MHz
Modulation type: pulsed
Exposure 2: 100 MHz
Modulation type: pulsed

Exposure 1

Main characteristics
Frequency 17 MHz
Modulation
Modulation type pulsed
Pulse width 60 ns
Exposure setup
Exposure source
  • two stainless steel electrodes, one rectangular and the other asymmetrically hexagonal
Setup cell suspension was placed in the channel between the electrodes (100 µm apart).
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 26 kV/cm unspecified unspecified - -

Exposure 2

Main characteristics
Frequency 100 MHz
Modulation
Modulation type pulsed
Pulse width 10 ns
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 65 kV/cm unspecified unspecified - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cell viability was not affected by nanosecond pulsed electromagnetic fields (nsPEFs). Propidium iodide uptake was not observed which indicates a viable cell membrane. Uptake of acridine orange leads to fluorescence change of the nucleus indicating a specific effect of nsPEFs of this organelle.

Study character:

Study funded by

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