Study type: Medical/biological study (experimental study)

Stimulation of capacitative calcium entry in HL-60 cells by nanosecond pulsed electric fields med./bio.

By: White JA, Blackmore PF, Schoenbach KH, Beebe SJ
Published in: J Biol Chem 2004; 279 (22): 22964-22972
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Aim of study (acc. to author)

To investigate the effects of nanosecond pulsed electric fields (nsPEFs) on the influx and release of calcium in human HL-60 cells in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Modulation type: pulsed
Exposure 2:
Modulation type: pulsed

Exposure 1

Main characteristics
Type
Waveform
Modulation
Modulation type pulsed
Pulse width 60 ns
Exposure setup
Exposure source
Setup cell suspension in a cuvette placed between the electrodes
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 400 kV/m minimum unspecified - 6.5 kV/cm, 10 kV/cm
electric field strength 15 kV/cm maximum unspecified - -

Exposure 2

Main characteristics
Type
Waveform
Modulation
Modulation type pulsed
Pulse width 60 ns
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 30 kV/cm unspecified unspecified - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Electric fields between 4 and 15 kV/cm with pulses of 60 ns duration induced an increase in intracellular calcium levels. This specific increase of calcium suggest that nsPEFs may affect either the plasma membrane or specific calcium ion channels therein.

Study character:

Study funded by

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