EMF-Portal | Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions

Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions med./bio.

By: Zmyslony M, Politanski P, Rajkowska E, Szymczak W, Jajte J

Published in: Bioelectromagnetics 2004; 25 (5): 324-328

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Aim of study (acc. to author)

Endpoint

Exposure

Exposure	Parameters
Exposure 1: 930 MHz Modulation type: CW Exposure duration: continuous for 5 or 15 min	power density: 5 W/m² unspecified SAR: 1.5 W/kg unspecified

Exposure 1

Main characteristics

Frequency	930 MHz
Type	electromagnetic field
Charakteristic	guided field
Exposure duration	continuous for 5 or 15 min

Modulation

Modulation type	CW

Exposure setup

Exposure source	GTEM cell
Setup	Plastic tubes (15 mm diameter) containing lymphocyte suspensions treated with 10 µg/ml ferrous chloride or distilled H₂O respectively were exposed in a tightly closed Plexiglas container (26 cm long, 17 cm diameter) which was placed in the GTEM cell. An identical container with unexposed samples was placed outside the GTEM cell (in the natural electromagnetic field).
Additional info	The experiment included four groups of lymphocytes: group I, not treated with ferrous ions and unexposed (control group); group II, not treated with ferrous ions and exposed; group III, treated with ferrous ions and unexposed; and group IV, treated with ferrous ions and exposed to electromagnetic radiation. Experiments were repeated twice, with 1 week interval between each replicate using rats from these same litter.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
power density	5 W/m²	unspecified	measured	-	-
SAR	1.5 W/kg	unspecified	calculated	-	-

Measurement and calculation details

The output power of the amplifier was measured using a directional coupler, a power meter and a thermocouple sensor. The field structure within the GTEM cell was investigated. Measurements were made at 5 cm intervals in both the horizontal and vertical planes using a single axis probe, an electric short dipole. In the volume occupied by the exposure system, field uniformity was better than 10%.

Methods Endpoint/measurement parameters/methodology

Investigated system:

intact cell/cell culture

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The data show that acute exposure does not affect the number of produced ROS. If, however, iron ions (FeCl₂) with final concentration 10 µg/ml) was added to the lymphocyte suspensions to stimulate ROS production, after both durations of exposure (5 and 15 min), the magnitude of fluorescence (ROS level during the experiment) was significantly greater in the irradiated cells.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

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