Study type: Medical/biological study (experimental study)

Cell-to-cell communication in response of E. coli cells at different phases of growth to low-intensity microwaves med./bio.

By: Shcheglov VS, Alipov ED, Belyaev IY
Published in: Biochimica et Biophysica Acta - General Subjects 2002; 1572 (1): 101-106
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Aim of study (acc. to author)

To investigate the effects of millimeter wave exposure (MMW) on DNA organisation of Escherichia coli at logarithmic and stationary growth. DNA organisation (i.e. different genome conformational stages (GCS) such as different degrees of DNA condensation) was analysed by the AVTD (anomalous viscosity time dependence) method.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 51.755 GHz
Modulation type: pulsed
Exposure duration: 10 min
Exposure 2: 51.755 GHz
Exposure duration: 10 min

Exposure 1

Main characteristics
Frequency 51.755 GHz
Charakteristic
  • guided field
Exposure duration 10 min
Modulation
Modulation type pulsed
Exposure setup
Exposure source
Additional info Control samples were kept simultaneously with the exposed samples outside the exposure system under the static magnetic field of 48 µT +/- 5 µT. VSWR did not exceed 1.4 in different points of the waveguide system.
Parameters
Measurand Value Type Method Mass Remarks
power density 10 W/m² mean calculated - -

Exposure 2

Main characteristics
Frequency 51.755 GHz
Charakteristic
  • guided field
Exposure duration 10 min
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 1 nW/cm² mean calculated - -

Reference articles

  • Belyaev IY et al. (1996): Resonance effect of millimeter waves in the power range from 10(-19) to 3 x 10(-3) W/cm² on Escherichia coli cells at different concentrations

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The temperature in cell suspensions was controlled during exposure and no heating was observed. Comparable cell densities were warranted. Stationary cells responded by significant increase in AVTD to MMW-exposure, whereas logarithmic cells revealed a significant decrease in AVTD. On the strength of the obtained data the authors suggest that cell-to-cell communication might be involved in the response of cells to weak MMW-exposure.

Study character:

Study funded by

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