To investigate if a microwave exposure is able to alter the heart rate of the frog Rana pipiens in vivo.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
1.25 GHz
Modulation type:
pulsed
Exposure duration:
intermittent, 10 min on/off, for up to 120 min
|
|
| Frequency | 1.25 GHz |
|---|---|
| Type | |
| Exposure duration | intermittent, 10 min on/off, for up to 120 min |
| Modulation type | pulsed |
|---|---|
| Pulse width | 5 µs |
| Repetition frequency | 1 Hz |
| Exposure source | |
|---|---|
| Distance between exposed object and exposure source | 90 cm |
| Chamber | The frogs were positioned on a polystyrene surface, restrained at arms and legs by plastic clips, and placed on a table made of RF-absorbing material approx. 90 cm below the open end of a standard gain horn antenna. |
| Setup | The frog's ECG was monitored with a pair of electrodes that were positioned perpendicular to the EF. The exposure pulses were synchronised with the rise of the R-wave or of the T-wave. |
| Sham exposure | A sham exposure was conducted. |
| Additional info | Alternating on and off periods were repeated until the heartbeat ceased. Generally the heart remained active for ten to twelve 10-min periods. Half of the exposures started with EMF off, the other half with EMF on. Two non-exposure conditions were used for control: In one condition, the preparation was shielded from the energy with a piece of RF-absorber, in the other one, the energy was delivered to a dummy load rather than to the horn antenna. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power density | 3 µW/cm² | mean | measured | - | - |
The heart rate of an in vivo preparation can be influenced by exposure to low-intensity pulsed electromagnetic radiation. The exposure has to be for more than one minute to reveal significant effects.
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