To establish a novel in vitro millimeter wave exposure system for monolayer cell cultures, which allows seeking for athermal biological effects under controlled temperature conditions.
As an indicator for possible biological effects the incorporation of radioactive labeled 3H-uridine and 3H-methionine into RNA and protein, respectively, was investigated by measuring of the optical densities of autoradiographes.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
65–75 GHz
Exposure duration:
continuous
|
|
|
Exposure 2:
42–48 GHz
Exposure duration:
continuous for 1 h
|
|
|
Exposure 3:
37–42 GHz
Exposure duration:
continuous for 1 h
|
|
| Frequency | 65–75 GHz |
|---|---|
| Type | |
| Exposure duration | continuous |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power density | 450 mW/cm² | average over time | measured | - | under the surface of the petri dish |
| Frequency | 42–48 GHz |
|---|---|
| Type | |
| Exposure duration | continuous for 1 h |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power | 180 mW | - | - | - | - |
| Frequency | 37–42 GHz |
|---|---|
| Type | |
| Exposure duration | continuous for 1 h |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power density | 320 mW/cm² | average over time | measured | - | under the surface of the petri dish |
Microwave-induced changes on RNA or protein synthesis did not occur when the temperature of cell cultures where set to 37°C by recirculation of the medium. In contrast, the cultures were affected (in such a way of marked heating, decline of macromolecular synthesis, and destruction of the monolayer) when recirculating and temperature control were shut down.
This website uses cookies to provide you the best browsing experience. By continuing to use this website you accept our use of cookies.
