Study type: Medical/biological study (experimental study)

The effect of 2450 MHz microwave radiation on histamine secretion by rat peritoneal mast cells med./bio.

By: Ortner MJ, Galvin MJ
Published in: Cell Biophys 1980; 2 (2): 127-138
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Aim of study (acc. to author)

To study the effect of microwave exposure on histamine secretion in mast cells.

Background/further details

Rat mast cells were obtained by peritoneal lavage, and histamine secretion was induced by using compound 48/80 in vitro. Histamine is stored in granuels inside the cell. The release of histamine requires a fusion of granules and cell membrane, this secretory process is, among other things, heat sensitive. Therefore the effect of cell heating was investigated additionally.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: 15, 30, 45, 60 min
  • SAR: 8.2 mW/g
Exposure 2: 2.45 GHz
Modulation type: CW
Exposure duration: continuous for 3 h

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration 15, 30, 45, 60 min
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup 10 mm x 75 mm culture tubes placed inside the waveguide
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 8.2 mW/g - calculated - -

Exposure 2

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration continuous for 3 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 41 mW/g - calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No change in morphological characteristics or viability of the cells could be observed. At 37°C irradiation does not effect the induced histamine release in cells treated with compound 48/80. At 44°C and SAR of 41.0 mW/g for 3h the histamine secretion was inhibited. It is not clear if this is caused due to direct thermal effects on the cell membrane or to electromagnetic field effects.

Study character:

Study funded by

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