Study type: Medical/biological study (experimental study)

Effects of 50 Hz magnetic field on cell cycle kinetics and the colony forming ability of budding yeast exposed to ultraviolet radiation med./bio.

By: Markkanen A, Juutilainen J, Lang S, Pelkonen J, Rytomaa T, Naarala J
Published in: Bioelectromagnetics 2001; 22 (5): 345-350
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Aim of study (acc. to author)

To investigate the effects of extremely low frequency magnetic fields on colony forming ability or cell cycle kinetics of ultraviolet radiation (UV) exposed yeast.

Background/further details

At various times after 10 minutes UV irradiation cells were collected for colony forming units and cell cycle measurements (0, 30, 60, 120, 240, 300, 360, and 420 min).The dose level for the UV radiation was 175 J/m². Exposure to UV (10 min) and magnetic field began simultaneously, and magnetic field exposure was continued to the end of experiment (up to 430 min).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: up to 430 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration up to 430 min
Exposure setup
Exposure source
  • Two 100 mm x 1300 mm rectangular coils
Setup A wooden exposure system, in which petri dishes along with the coils were situated at the upper part and a UV lamp placed underneath the petri dishes.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 120 µT mean - - +/- 5 µT
Additional parameter details

Background MF in the incubation room was 0.05 µT. UV radiation of 175 J/m².

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data on cell cycle kinetics suggest different delay times in (UV + magnetic field) exposed yeast cells compared with only UV exposed yeast cells during the first cell cycle. The effect of magnetic field on cell cycle kinetics was seen only at one time point (at 60 min after UV irradiation).
The effect of the magnetic field exposure on the colony forming ability of the UV irradiated yeast cells was statistically significant 420 min after UV irradiation (fewer colony forming units). Moreover, at other time points after UV irradiation, there were fewer colony forming units in "UV+magnetic field" exposed populations than in only UV exposed yeast cells, although the differences were not statistically significant.
These findings could indicate that magnetic field exposure in conjunction with UV may have some effects on yeast cell survival or growth.

Study character:

Study funded by

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