To study the influence of low frequency, low amplitude magnetic fields on the diffusion processes in enzyme-loaded liposomes. The influence on the influx rate of the substrate p-nitrophenyl acetate into carbonic anhydrase-loaded liposomes was studied.
Cationic liposomes containing dipalmitoylphosphatidylcholine, cholesterol, and charged lipid stearylamine at different molar ratios (6:3:1 or 5:3:2) were used to evaluate the involvement of charged lipid (i.e. stearylamine) in the interaction with electromagnetic fields.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
4–21 Hz
Exposure duration:
continuous for 60 min
|
|
| Exposure source |
|
|---|---|
| Setup | triaxial coils systems with a large isofield region in the center; aliquots placed in 3 cm³ silica cuvettes in this isofield region |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 75 µT | maximum | - | - | 0-75 µT AC |
| magnetic flux density | 75 µT | maximum | - | - | 0-75 µT DC |
The data support the view that exposure to combined low-intensity AC-DC magnetic fields affects the charges of lipids on liposome surface leading to membrane permeability changes. After 60 min of exposure to 7 Hz sinusoidal and parallel static (50 µT) magnetic fields the enzyme activity, as a function of increased diffusion rate of p-nitrophenyl acetate, rose from 17 +/- 3% to 80 +/- 9% in the 5:3:2 liposomes. This effect was dependent on the stearylamine concentration in the liposomes. No enzyme leakage was found.
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