Study type: Medical/biological study (experimental study)

DNA Strand Breaks in Fibroblasts Exposed to a 50-Hz Magnetic Field med./bio.

By: Nakayama M, Hondou T, Miyata H
Published in: Proceedings of the 12th Asia Pacific Physics Conference (APPC12), 2013. JPS, 2014: p. 012057; ISBN 978-4-89027-101-6
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Aim of study (acc. to author)

The genotoxic effect of exposure of mouse fibroblasts to 50 Hz magnetic fields should be investigated.

Background/further details

It should be examined whether previous results on DNA strand breaks (Ivancsits et al. 2002, Ivancsits et al. 2003, Ivancsits et al. 2003, Ivancsits et al. 2005) are reproducible.
For each exposure condition and duration, a separate sham exposure was conducted.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: intermittent (on for 5 min, off for 10 min) for 15, 18 and 24 hours
Exposure 2: 50 Hz
Exposure duration: intermittent (on for 5 min, off for 10 min) for 15, 18 and 24 hours
Exposure 3: 50 Hz
Exposure duration: intermittent (on for 5 min, off for 10 min) for 15, 18 and 24 hours
Exposure 4: 50 Hz
Exposure duration: continuous for 15, 18 and 24 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent (on for 5 min, off for 10 min) for 15, 18 and 24 hours
Exposure setup
Exposure source
  • not specified
Chamber incubator
Setup two identical sets of exposure systems in two incubators of the same type, so exposure and sham exposure could be run simultaneously
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 100 µT - - - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent (on for 5 min, off for 10 min) for 15, 18 and 24 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT - - - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent (on for 5 min, off for 10 min) for 15, 18 and 24 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1,000 µT - - - -

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 15, 18 and 24 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT - - - -

Reference articles

  • Miyata H et al. (2015): Analysis of gene expression in human umbilical vein endothelial cells exposed to a 50-Hz magnetic field

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

At 100 µT intermittent exposure, there was no significantly difference in DNA strand breaks between exposed and sham exposed cells at any point in time. At 500 µT intermittent exposure, there were significantly more DNA strand breaks in exposed cells compared to sham exposed cells after 18 h and 24 hours. At 1000 µT intermittent exposure, there were significantly more strand breaks after 18 hours only compared to sham exposed cells.
In continuous exposure conditions, there were significantly more DNA strand breaks in cells exposed to 500 µT compared to sham exposed cells after 18 hours only.
The authors conclude that exposure of mouse fibroblasts to 50 Hz magnetic fields might have a genotoxic effect.

Study character:

Study funded by

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