Study type: Medical/biological study (experimental study)

Effect of extremely low frequency electromagnetic field on brain histopathology of Caspian Sea Cyprinus carpio med./bio.

Published in: Electromagn Biol Med 2017; 36 (1): 31-38

Aim of study (acc. to author)

The effects of exposure of carps to different 50 Hz magnetic fields of varying strength on brain histopathology should be investigated.

Background/further details

200 healthy common carps (Cyprinus carpio) were divided into the following groups (n=10 each): exposure to a magnetic field of 1) 0.1 mT for 0.5 hours, 2) 0.1 mT for 1 h, 3) 1 mT for 0.5 h, 4) 1 mT for 1 hour, 5) 3 mT for 0.5 h, 6) 3 mT for 1 hour, 7) 5 mT for 0.5 h, 8) 5 mT for 1 h, 9) 7 mT for 0.5 h, 10) 7 mT for 1 h. For each group, a separate sham exposure group was used.
After (sham) exposure, fish were returned to their aquaria for 2 weeks.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 0.5 hours
Exposure 2: 50 Hz
Exposure duration: continuous for 1 hour
Exposure 3: 50 Hz
Exposure duration: continuous for 0.5 hours
Exposure 4: 50 Hz
Exposure duration: continuous for 1 hour
Exposure 5: 50 Hz
Exposure duration: continuous for 0.5 hours
Exposure 6: 50 Hz
Exposure duration: continuous for 1 hour
Exposure 7: 50 Hz
Exposure duration: continuous for 0.5 hours
Exposure 8: 50 Hz
Exposure duration: continuous for 1 hour
Exposure 9: 50 Hz
Exposure duration: continuous for 0.5 hours
Exposure 10: 50 Hz
Exposure duration: continuous for 1 hour

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 0.5 hours
Exposure setup
Exposure source
Chamber acrylic experimental cubes (15 cm Œ 8 cm Œ 6 cm) filled with aquarium water and placed inside the coil
Setup cylindrical coil, 50 cm in length, with inner diameter of 9.6 cm and outer diameter 11.5 cm, made of 980 turns of 2.5 mm diameter enameled copper wire; fish were positioned inside the coil on an elevated platform to ensure all of them received the same magnetic field intensity; the coil was located in an east-west direction and the magnetic field was parallel to the long axis of fish body; temperature was maintained at 16°C
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT - calculated - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 hour
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT - calculated - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 0.5 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - calculated - -

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 hour
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - calculated - -

Exposure 5

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 0.5 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 3 mT - calculated - -

Exposure 6

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 hour
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 3 mT - calculated - -

Exposure 7

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 0.5 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - calculated - -

Exposure 8

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 hour
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - calculated - -

Exposure 9

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 0.5 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 7 mT - calculated - -

Exposure 10

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 1 hour
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 7 mT - calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No pathologic changes were observed at magnetic flux densities of 0.1 and 1 mT regardless of the exposure duration (groups 1-4).
Starting with a magnetic flux density of 3 mT for 0.5 h (group 5), brain slices showed increasing histopathological changes with increasing magnetic flux density and exposure duration.
At 3 mT (groups 5 and 6), signs of gliosis were found. At 5 mT (groups 7 and 8), lesions increased, showing infiltration of lymphocytic cells and edema. Finally at 7 mT (groups 9 and 10), advanced forms of findings of the previous groups and necrosis were found.
The authors conclude that exposure of carps to a 50 Hz magnetic field of 3 mT and more might induce histopathological changes in the brain depending on the magnetic flux density and the exposure duration.

Study character:

Study funded by

Related articles